molecular characterization and phylogenetic congruence of hydropsyche sciligra (tricoptera: hydropsychidae) using mitochondrial and nuclear markers
نویسندگان
چکیده
background: caddisflies have significant roles in freshwater ecosystems. morphological identification is the major impediment in accurate species identification of hydropsychids. mitochondrial and nuclear markers are suitable for molecular systematics of these group of arthropods. methods: trichopteran specimens of lavasan district in northeastern tehran, iran were collected in 2012, and described using the morphological and molecular characters of mitochondrial cytochrome c oxidase subunit i (mt-coi) and three expansion fragments of large subunit (lsu) nuclear ribosomal dna (28s rdna) d1, d2, and d3. the resemblance of the specimen sequences was obtained by conducting blast searches against the genbank database and by using simple maximum likelihood clustering using coi, d1, d2, d3, and combination of d1-d2-d3 sequence data sets. results: based on morphological traits the specimens were resembled to hydropsyche sciligra however there were no its counterpart sequences in the genbank. due to lack of unique group of data set for each gene fragment, the specimens were associated with different taxa on molecular phylograms. the sequence contents of the coi, d1, d2, d3, and d1-d3 regions clustered h. sciligra with h. brevis , h. angustipennis , h. occidentalis , h. hedini , h. grahami , and h. longifurca / h. naumanni, respectively. conclusion: phylogenies obtained from combination of d1-d3 showed the highest bootstrap values for most of clades suggesting that long lsu-rdna potentially is more useful for understanding phylogenetic relationships of caddisflies. a large-scale molecular and zoogeographic study on trichopteran species is suggested to revise and to develop the current knowledge of the caddisfly fauna and distributions in the country.
منابع مشابه
Molecular Characterization and Phylogenetic Congruence of Hydropsyche sciligra (Tricoptera: Hydropsychidae) Using Mitochondrial and Nuclear Markers
BACKGROUND Caddisflies have significant roles in freshwater ecosystems. Morphological identification is the major impediment in accurate species identification of Hydropsychids. Mitochondrial and nuclear markers are suitable for molecular systematics of these group of arthropods. METHODS Trichopteran specimens of Lavasan District in northeastern Tehran, Iran were collected in 2012, and descri...
متن کاملon the relationship between using discourse markers and the quality of expository and argumentative academic writing of iranian english majors
the aim of the present study was to investigate the frequency and the type of discourse markers used in the argumentative and expository writings of iranian efl learners and the differences between these text features in the two essay genres. the study also aimed at examining the influence of the use of discourse markers on the participants’ writing quality. to this end the discourse markers us...
15 صفحه اولpreparation and characterization of new co-fe and fe-mn nano catalysts using resol phenolic resin and response surface methodology study for fischer-tropsch synthesis
کاتالیزورهای co-fe-resol/sio2و fe-mn-resol/sio2 با استفاده از روش ساده و ارزان قیمت همرسوبی تهیه شدند. از رزین پلیمری resol در فرآیند تهیه کاتالیزور استفاده شد.
synthesis and characterization of some macrocyclic schiff bases
ماکروسیکلهای شیف باز از اهمیت زیادی در شیمی آلی و دارویی برخوردار می باشند. این ماکروسیکلها با دارابودن گروه های مناسب در مکانهای مناسب می توانند فلزاتی مثل مس، نیکل و ... را در حفره های خود به دام انداخته، کمپلکسهای پایدار تولید نمایند. در این پایان نامه ابتدا یک دی آلدئید آروماتیک از گلیسیرین تهیه می شود و در مرحله بعدی واکنش با دی آمینهای آروماتیک و یا آلیفاتیک در رقتهای بسیار زیاد منجر به ت...
15 صفحه اولsynthesis of amido alkylnaphthols using nano-magnetic particles and surfactants
we used dbsa and nano-magnetic for the synthesis of amido alkylnaphtols.
15 صفحه اولMolecular Characterization of Zhumeria majdea Iranian Germplasms Using ISSR Markers
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were colle...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of arthropod-borne diseasesجلد ۲۰۱۵، شماره ۱۰، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023